AnyDetect

AnyDetect

Portable Real-Time
Isothermal Amplifier

With the development of AnyDetect, a genetic test that used to take several days for a definitive diagnosis by sending it to a specialized laboratory, even unskilled workers can easily test it on the spot.

DNA/RNA isothermal amplification device employing LAMP method

Any Detect is a general-purpose equipment that can apply all isothermal amplification technologies such as LAMP and RPA, and it is equipped with an amplification reaction unit and a detection unit to interpret the results in real time.

LAMP

Steps of temperature adjustment

isothermal amplification
(Single Step)

PCR

Complicated
(Thermal cycling)

Detection Speed

〜30min.

〜120min.

Sensitivity to Specimen Inhibitors

Less effect of inhibitors

susceptible to the influence of inhibitors

Necessity of amplification confirmation device

Not required
(check with Real Time)

Required dyeing reagent or
gel electrophoresis

Battery-powered portable detection device

    It is equipped with a rechargeable battery (5,200mAh), so it can operate for more than 6 hours on a single charge.

6 hours

Compact design

Compact design optimized for ultra light weight (approx. 850 g)

Simultaneous detection of 8 channels

8 specimens can be set, allowing simultaneous testing of 8 samples.

High on-site availability

As it is powered by a rechargeable battery, it has portability and fieldability, so it can be expected to quickly respond to various situations such as livestock farms, food processing sites, and public facilities and environmental pollution investigations.

fig1.png

노로바이러스

장관출혈성대장균

(O-157)

​캠필로박터

살모넬라

조류독감

Detection example

Enterohemorrhagic E. coli O-157

Positive
Negative

Shows the results of testing positive samples and negative controls with our LAMP kit that can detect O157 E. coli. In the positive sample, an amplification signal appeared from around 23 minutes (red arrow), whereas in the negative control group, no amplification signal was formed until the reaction was completed (blue arrow).

Campylobacter

Our LAMP kit test results are shown for standard samples serially diluted with Campylobacter bacteria. For 1,000 copies, the amplification signal appears from about 25 minutes after the start of the reaction, for 500 copies, around 32 minutes, and for 100 copies, the amplification signal appears around 39 minutes. In particular, it can be seen that the amplification signal is confirmed around 45 minutes even when reacting with a trace sample of 10 copies.

1,000 copy
500 copy
100 copy
10 copy

Interlocking with PC and smartphone

Can check detailed measurement results in real time with a PC program and a smartphone app.

Micro 5pin

Smartphone
(IOS / Android)

PC

(Windows 8/10)

FAQ

Q. What kind of amplification technology can AnyDetect apply to?

A. Any isothermal amplification method that reacts at a temperature within the operating range of 40-80 degrees is applicable. In particular, we are providing various test kits for the LAMP test method.

Q. How should the sample be prepared?

A. Compared to other test methods, our LAMP kit is more tolerant of inhibitors and can be applied without purification. However, to ensure high specificity and sensitivity, it is recommended to use DNA/RNA separated/purified by column or bead method.

Q. How long does detection take?

The time taken for the amplification reaction can be fast or slow because it is affected by various factors such as the amount of the sample, the purity of the sample, and the external environment. The LAMP kits we provide are optimized to confirm results, typically within 10 to 40 minutes.

Q. How can the positive result of the target be confirmed?

When an amplification reaction occurs in a positive sample through our LAMP kit, the degree of turbidity becomes severe, and the presence or absence of positive/negative is checked through the detection unit of AnyDetect, which can detect turbidity in real time.

Q. What is the difference between PCR test and LAMP test?

PCR requires thermal cycling, whereas LAMP reacts at a single temperature, allowing for miniaturization of the device and good portability. While PCR increases amplification products by a factor of two for each reaction cycle, LAMP produces more amplification products in the same time as there is no limit to this. In the case of the actual LAMP test, it is often possible to determine the result within 15 minutes. In addition, the LAMP test method has the advantage of being less restrictive in sample preparation because it is more tolerant of reaction inhibitors than PCR.

Specification

Item

Contents

Test Wells

200㎕ 8 tubes PCR Stripe

Optical Wavelength

650nm

Reaction Control Temp.

50℃ ~ 80℃

Temp. Difference

±0.5℃

Scan Interval

10 second

Interface

Bluetooth (Mobile Phone : Android, iOS) 

Micro USB 5pin (USB Type A PC/Notebook)

Display

LCD 4.3“

Storage

SD Card

Power

AC/DC Adapter(Charger) : 100~240Vac 50/60Hz, 9Vdc/3A 

Battery Life : Min. 6 hours @60℃ vs 25℃ ambient

Dimension

150mm x 169mm x 89mm

Weight

~900g

Operating Environment

Temperature: 10℃ ~ 40℃ / Humidity: 10% ~ 80%

Storage Environment

Temperature: 5℃ ~ 50℃ / Humidity: 10% ~ 80%